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BILE SALT TBL POWDER (STD)
Bile salt (STD) TBL powder is developed by Titan Biotech Ltd. specifically for microbiological culture media, which is more purified and light in colour. Bile salts are bile acids compounded with a cation, usually sodium. The salts of taurocholic acid and glycocholic acid represent bile salts. This Sodium glycotaurocholate are used as selective agent mainly to inhibit the gram positive organisms and non-intestinal organisms.
• It is usually added as ingredient in microbiological culture media, such as in MacConkey Agar and Broth, SS Agar, Violet Red Bile Agar, Brilliant Green Bile Broth and E.E Broth.
• Bile salt increases the selectivity of the medium by inhibiting gram positive microbes.
• This Bile salt entered in the growth pattern and altered fermentation of lactose.
• It does not allow growing faecal Staphylococci, Pneumococcus and Streptococcus.
Greenish yellow colour free flowing powder having characteristic odour and smell.
Solubility (2% soln. at 25oC)
Completely soluble in distilled water and alcohol, clear.
Clarity (2% Soln. at 121 ºC.)*
Absolute clear solution. No ppt.
pH (2% Soln. at 25 ºC)
6.0 - 8.0
Loss on drying ( at 105 ºC)
NMT - 5.0%
NMT - 20.0%
NLT - 45.0%
Total viable count
Yeast & molds
GROWTH PROMOTION TEST
*Prepare a sterile medium with 10 gm peptone, 10 gm lactose, 20 gm Bile salt (STD) TBL & 0.0133 gm brilliant green in 1Liter distilled water. Adjust pH to 7.2 ± 0.2. Inoculate with the bellow mentioned organisms & incubate at 35 – 370C for 48 hrs.
Good – Luxuriant/ bile ppt.
Good - Luxuriant
Std Packing : 25kg, HDPE drum
Best Before : 36 Months
Storage : Hygroscopic by nature, store the material below 25ºC, protect from direct sunlight.