Bile Salt Powder

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Bile Salt Powder

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Product Code : Bacteriological Grad

Brand Name : Bacteriological Grad

Product Description

Description:
Bile salt constitutes pure substances, sodium conjugates of glycotaurocholate, and those are used as selective agents mainly to inhibit gram-positive organisms, non-intestinal organisms and spore formers while permitting the growth of gram-negative enterics. Bile salt inhibits Enterococcus and Staphylococcus species.
Use:
Bile salt powder of Titan Biotech Ltd. is developed specifically for biotechnology and culture media applications.
• Bile salt transformation and degradation is of high ecological relevance and also essential for the biotechnological production of drugs.
• Bile salt entered in the growth pattern and altered fermentation of lactose.
PHYSICAL PARAMETERS

S.L. No.	PRODUCT PARAMETERS	SPECIFICATION
1.	Appearance	Greenish to yellowish colour free flowing powder, no pungent smell.
2.	Solubility (2% soln. at 25oC)	Soluble in distilled water and alcohol.
3.	Clarity (2% Soln. at 121 ºC.)*	Clear solution. No ppt.

* - After Autoclave
CHEMICAL PARAMETERS

S.L. No.	PRODUCT PARAMETERS	SPECIFICATION
1.	pH (2% Soln. at 25 ºC)	6.5 - 8.0
2.	Loss on drying ( at 105 ºC)	NMT - 5.0%
3.	Total Ash	NMT - 20.0%
4.	Cholic Acid (as Sodium Cholate)	NLT - 45.0%

MICROBIAL PARAMETERS

S.L. No.	PRODUCT PARAMETERS	SPECIFICATION
1.	Total viable count	NMT- 10,000cfu/gm
2.	Escherichia coli	Negative
3.	Salmonella spp.	Negative
4.	Staphylococcus aureus	Negative
5.	Yeast & molds	100/gm

GROWTH PROMOTION TEST
Cultural Characteristic observed in MacConkey agar with 0.5% Bile salt Powder after incubation at 35-37°C for 18-24 hours.

S.L. No.	MICROORGANISMS	ATCC	GROWTH	RECOVERY
1.	Staphylococcus aureus	25923	Inhibited	-----------
2.	Escherichia coli	25922	Luxuriant / bile ppt.	>= 50%
3.	Enterococcus faecalis	29212	Fair-Good	>= 50%
4.	Salmonella typhimurium	14028	Luxuriant	>= 50%