HPLC Column

Name: HPLC Column
Brand: CHINTAN ENTERPRISE
Price: 1000 INR
Availability: InStock

Price 1000 INR/ Unit

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MOQ : 1 Unit

HPLC Column Specification

Temperature Resistance
Up to 60C

Shape
Cylindrical

Accuracy
High Separation Efficiency

Features
High efficiency, durable construction, precise separation

Type
HPLC Column

Dimension (L*W*H)
250 mm 4.6 mm (I.D.)

Weight
1.5 Kilograms (kg)

Equipment Type
Analytical Column

Equipment Materials
Stainless Steel

Material
Stainless Steel with Silica Packing

Application
High Performance Liquid Chromatography (HPLC)

Column Chemistry
C18 (Octadecyl Silane bonded silica)

PH Range
2-8

Particle Size
5 m

Packing Material
High Purity Spherical Silica

Maximum Pressure
6000 psi

Compatibility
Universal with most HPLC systems

Connector Type
1/16

Pore Size
120 A

Endcapped
Yes

HPLC Column Trade Information

Minimum Order Quantity
1 Unit

Supply Ability
1 Unit Per Week

Delivery Time
1 Week

HPLC Column About

HAMILTON HPLC COLUMN

application(s)	HPLC: suitable
L I.D.	10 cm 4.1 mm
matrix	PS-DVB particle platform
matrix active group	ammonium ion, quaternary phase
particle size	10 m
pore size	100
mode of chromatography	anion exchange

Exceptional Separation and Efficiency

Engineered for high performance, this analytical HPLC column provides outstanding separation efficiency due to its optimal 5 m particle size and high purity spherical silica packing. The C18 bonded phase offers maximum retention for a broad spectrum of analytes, ensuring highly reproducible results across different analytical sessions.

Robust Construction and Compatibility

The column's stainless steel body and endcapped C18 chemistry provide durability and stability even under rigorous pressure (up to 6000 psi) and temperature (up to 60C) conditions. Standard 1/16" fittings ensure seamless integration with most HPLC systems, making it a versatile choice for laboratories and process industries alike.

Versatile Applications Across Industries

Capable of reliable operation within a pH range of 2-8, this column is suited for pharmaceutical, biochemical, and environmental analyses. Its high durability and compatibility make it an excellent solution for laboratories seeking consistent results, whether for routine quality control or advanced research applications.

FAQ's of HPLC Column:

Q: How should I properly install the HPLC column to ensure optimal performance?

A: To install the column, connect it using the universal 1/16" fittings, ensuring tight, leak-free junctions. Always flush the HPLC system with the appropriate solvent before connecting the column to prevent contamination and enhance column efficiency.

Q: What types of samples can be separated using this C18 HPLC column?

A: This analytical column is engineered for separating a wide variety of non-polar to moderately polar compounds, making it suitable for pharmaceutical, biochemical, and environmental samples requiring high separation efficiency.

Q: When is it advisable to replace the HPLC column?

A: Column replacement is recommended when you observe significant loss in separation efficiency, increased backpressure, or abnormal peak shapes, indicating column fouling or degradation of the C18 bonded phase.

Q: Where can this HPLC column be used effectively?

A: This column is well-suited for laboratories, research institutions, pharmaceutical companies, and quality control departments that utilize high-performance liquid chromatography systems for routine and advanced analysis.

Q: What is the process for conditioning this column before its first use?

A: Before initial use, flush the column with 10-20 column volumes of the starting mobile phase at a flow rate recommended in the accompanying manual, ensuring the removal of storage solvents and equilibrating the stationary phase for precise separation.

Q: How does the endcapped C18 chemistry benefit chromatographic analysis?

A: The endcapping process minimizes unwanted secondary interactions, resulting in sharper peaks and improved reproducibility, especially for basic analytes or compounds prone to tailing, thus enhancing overall chromatographic quality.

Q: What advantages does the high purity spherical silica packing provide?

A: High purity spherical silica offers improved flow characteristics, greater mechanical stability, and consistent batch-to-batch performance, leading to highly accurate and reproducible separation results.

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